Resveratrol and clofarabine induces a preferential apoptosis-activating effect on malignant mesothelioma cells by Mcl-1 down-regulation and caspase-3 activation
- Abstract
- We previously demonstrated that resveratrol and clofarabine elicited a marked cytotoxicity on malignant mesothelioma (MM) MSTO-211H cells but not on the corresponding normal mesothelial MeT-5A cells. Little is known of the possible molecules that could be used to predict preferential chemosensitivity on MSTO-211H cells. Resveratrol and clofarabine induced down-regulation of Mcl-1 protein level in MSTO-211H cells. Treatment of cells with cycloheximide in the presence of proteasome inhibitor MG132 suggested that Mcl-1 protein levels were regulated at the post-translational step. The siRNA-based knockdown of Mcl-1 in MSTO-211H cells triggered more growth-inhibiting and apoptosis-inducing effects with the resultant cleavages of procaspase-3 and its substrate PARP, increased caspase-3/7 activity, and increased percentage of apoptotic propensities. However, the majority of the observed changes were not shown in MeT-5A cells. Collectively, these studies indicate that the preferential activation of caspase cascade in malignant cells might have important applications as a therapeutic target for MM.
- All Author(s)
- Y. J. Lee
; S. H. Lee
- Issued Date
- 2015
- Type
- Article
- Keyword
- Chemosensitivity; Clofarabine; MeT-5A; MSTO-211H; Resveratrol
- Publisher
- 생화학분자생물학회
- ISSN
- 1976-6696
- Citation Title
- BMB Reports
- Citation Volume
- 48
- Citation Number
- 3
- Citation Start Page
- 166
- Citation End Page
- 171
- Language(ISO)
- eng
- DOI
- 10.5483/bmbrep.2015.48.3.105
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/1571
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