The effect of post-treatment N-acetylcysteine in LPS-induced acute lung injury of rats
- Abstract
- BACKGROUND: Oxidation plays an important role in acute lung injury. This study was conducted in order to elucidate the effect of repetitive post-treatment of N-acetylcysteine (NAC) in lipopolysaccaride (LPS)-induced acute lung injury (ALI) of rats. METHODS: Six-week-old male Sprague-Dawley rats were divided into 4 groups. LPS (Escherichia coli 5 mg/kg) was administered intravenously via the tail vein. NAC (20 mg/kg) was injected intraperitoneally 3, 6, and 12 hours after LPS injection. Broncho-alveolar lavage fluid (BALF) and lung tissues were obtained to evaluate the ALI at 24 hours after LPS injection. The concentration of tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) were measured in BALF. Nuclear factor κB (NF-κB), lipid peroxidation (LPO), and myeloperoxidase (MPO) were measured using lung tissues. Micro-computed tomography (micro-CT) images were examined in each group at 72 hours apart from the main experiments in order to observe the delayed effects of NAC. RESULTS: TNF-α and IL-1β concentration in BALF were not different between LPS and NAC treatment groups. The concentration of LPO in NAC treatment group was significantly lower than that of LPS group (5.5±2.8 nmol/mL vs. 16.5±1.6 nmol/mL) (p=0.001). The activity of MPO in NAC treatment group was significantly lower than that of LPS group (6.4±1.8 unit/g vs. 11.2±6.3 unit/g, tissue) (p<0.048). The concentration of NF-κB in NAC treatment group was significantly lower than that of LPS group (0.3±0.1 ng/µL vs. 0.4±0.2 ng/µL) (p=0.0001). Micro-CT showed less extent of lung injury in NAC treatment than LPS group. CONCLUSION: After induction of ALI with lipopolysaccharide, the therapeutic administration of NAC partially attenuated the extent of ALI through the inhibition of NF-κB activation.
- All Author(s)
- J. S. Choi
; H. S. Lee
; K. H. Seo
; J. O. Na
; Y. H. Kim
; S. T. Uh
; C. S. Park
; M. H. Oh
; S. H. Lee
; Y. T. Kim
- Issued Date
- 2012
- Type
- Article
- Keyword
- Acetylcysteine; Acute Lung Injury; Antioxidants
- Publisher
- 대한결핵및호흡기학회
The Korean Academy of Tuberculosis and Respiratory Diseases
- ISSN
- 0378-0066
; 1738-3536
; 2005-6184
- Citation Title
- Tuberculosis and respiratory diseases
- Citation Volume
- 73
- Citation Number
- 1
- Citation Start Page
- 22
- Citation End Page
- 31
- Language(ISO)
- eng
- DOI
- 10.4046/trd.2012.73.1.22
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/1929
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