Gene profile of fibroblasts identify relation of CCL8 with idiopathic pulmonary fibrosis
- Abstract
- Background: Idiopathic pulmonary fibrosis (IPF) is characterized by the complex interaction of cells involved in chronic inflammation and fibrosis. Global gene expression of a homogenous cell population will identify novel candidate genes.
Methods: Gene expression of fibroblasts derived from lung tissues (8 IPF and 4 controls) was profiled, and ontology and functional pathway were analyzed in the genes exhibiting >2 absolute fold changes with p-values < 0.05. CCL8 mRNA and protein levels were quantified using real-time PCR and ELISA. CCL8 localization was evaluated by immunofluorescence staining.
Results: One hundred seventy eight genes differentially expressed and 15 genes exhibited >10-fold change. Among them, 13 were novel in relation with IPF. CCL8 expression was 22.8-fold higher in IPF fibroblasts. The levels of CCL8 mRNA and protein were 3 and 9-fold higher in 14 IPF fibroblasts than those in 10 control fibroblasts by real-time PCR and ELISA (p = 0.022 and p = 0.026, respectively). The CCL8 concentrations in BAL fluid was significantly higher in 86 patients with IPF than those in 41 controls, and other interstitial lung diseases including non-specific interstitial pneumonia (n = 22), hypersensitivity pneumonitis (n = 20) and sarcoidosis (n = 19) (p < 0.005, respectively). Cut-off values of 2.29 pg/mL and 0.43 pg/mL possessed 80.2 and 70.7% accuracy for the discrimination of IPF from NC and the other lung diseases, respectively. IPF subjects with CCL8 levels >28.61 pg/mL showed shorter survival compared to those with lower levels (p = 0.012). CCL8 was expressed by α-SMA-positive cells in the interstitium of IPF.
Conclusions: Transcriptome analysis identified several novel IPF-related genes. Among them, CCL8 is a candidate molecule for the differential diagnosis and prediction of survival.
- All Author(s)
- J. U. Lee
; H. S. Cheong
; E. Y. Shim
; D. J. Bae
; H. S. Chang
; S. T. Uh
; Y. H. Kim
; J. S. Park
; B. Lee
; H. D. Shin
; C. S. Park
- Issued Date
- 2017
- Type
- Article
- Keyword
- CCL8; Gene expression; IPF; Transcriptome
- Publisher
- BioMed Central Ltd
- ISSN
- 1465-9921
; 1465-993X
- Citation Title
- Respiratory Research
- Citation Volume
- 18
- Citation Number
- 1
- Citation Start Page
- 3
- Citation End Page
- 3
- Language(ISO)
- eng
- DOI
- 10.1186/s12931-016-0493-6
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/2083
- 공개 및 라이선스
-
- 파일 목록
-
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.