Effect of Central Losartan on DOCA-Salt Hypertension Rats
- Abstract
- BACKGROUND: The purpose of this study was to investigate whether brain AT1 receptor stimulation contributes as a hypertensive mechanism to deoxycorticosterone acetate (DOCA)-salt hypertension.
METHODS: 1) Acute injection:Losartan (1 mg/4 uL) or artificial cerebrospinal fluid (aCSF) was injected into the lateral cerebral ventricle (icv) of conscious control uninephrectomized Wistar rats or rats with DOCA-salt at 2 or 4 weeks, and mean arterial pressure (MAP) and heart rates (HR) were recorded. 2) Chronic injection:Using osmotic minipump, losartan (1 mg/kg/d) or aCSF was injected to a sham group or three DOCA-salt rat groups [icv-aCSF, icv-losartan, sc-losartan (subcutaneous) groups] for 4 weeks, after which the MAP and HR were recorded in addition to the weights of the left (LV) and right ventricles (RV) and kidneys.
RESULTS: 1) Acute injection: In rats treated with DOCA-salt, resting MAP significantly increased compared to the control group [144±6 mmHg (2 weeks), 170±5 mmHg (4 weeks) vs 115-120 mmHg (controls)]. MAP decreased significantly (2 weeks, 4 weeks) at 4, 8, 24 hours after icv injection of losartan to the level of the control group. 2) Chronic injection: The general trend showed that MAP decreased more in the icv-losartan group than in the icv-aCSF group (127±15.2 mmHg vs 141.1±5.5 mmHg, p=0.0578). In all DOCA-salt groups, no differences in RV weight were found. In the icv-aCSF and sclosartan groups, the kidney weight increased compared to the control group, but there was no difference in LV and kidney weight between the icv-losartan group and the control group.
CONCLUSION: Normalization of MAP after acute or chronic icv administration of the AT1 receptor antagonist suggests that the stimulation of the brain AT1 receptor plays a significant role in the development and maintenance of hypertension in the DOCA-salt hypertensive rat model. Losartan icv injection appeared to have a protective effect on the heart and kidney.
- All Author(s)
- S. J. Lee
; C. G. Park
; J. S. Park
; E. J. Kim
; S. H. Shin
; D. G. Jin
; J. C. Ahn
; H. S. Seo
; D. J. Oh
; Y. M. Ro
- Issued Date
- 2004
- Type
- Article
- Keyword
- Losartan; Angiotensin II; Receptor, angiotensin, type 1; Brain; DOCA-salt hypertension
- Publisher
- 대한심장학회
Korean Society of Cardiology
- ISSN
- 1225-164X
- Citation Title
- 순환기
The Korean circulation journal
- Citation Volume
- 34
- Citation Number
- 1
- Citation Start Page
- 84
- Citation End Page
- 91
- Language(ISO)
- eng
- DOI
- 10.4070/kcj.2004.34.1.84
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/3047
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