증폭된 조혈전구세포에서 Fas 항원 및 Bax의 발현과 아포토시스
- Alternative Title
- The Expression of Fas antigen and Bax and Apoptosis in Ex Vivo Expanded Hematopoietic Progenitor Cells
- Abstract
- BACKGROUND:
During ex vivo expansion of cord blood (CB) CD34+ cells, differentiation of the expanded cells happened and hematopoietic potential of the progenitor cells decreased. In this study, we evaluate the effect of the expression of Fas antigen, Bcl-2, and Bax on CD34+ or AC133+ hematopoietic progenitor cells during ex vivo expansion.
METHODS : CD34+ and AC133+ cells isolated from human CB were cultured in serum free medium supplemented with several cytokines for 7 days. After expansion culture, we re isolated CD34+ and AC133+ cells and compared the numbers of granulocyte-macrophage colony- forming units (CFU-GM) and granulocyte, erythrocyte, monocyte, and macrophage colony-forming units (CFU-GEMM), and expression of Fas antigen, Bcl-2, and Bax with unexpanded cells.
RESULTS : CFU-GM was expanded 23.94 fold in CD34+ cells and 15.22 fold in AC133+ cells at day 7 of culture but CFU-GEMM was not expanded. The expression of Fas antigen and Bax was 7.44% and 2.75%, respectively, in fresh isolated CD34+ cells and increased to 19.71 % and 33.67%, respectively, in expanded CD34+ cells at day 7 culture, but Bcl-2 was not changed. In case of AC133+ cells, the expression of Fas antigen and Bax were also increased from 5.87% and 6.19% to 24.85% and 22.83%, respectively, and Bcl-2 was slightly decreased. Apoptosis was not changed in CD34+ cells and AC133+ cells during ex vivo expansion.
CONCLUSION :
These results indicate that the nature of expansion was similar between CD34+ and AC133+ cells, and expression of Fas antigen and Bax increased on CD34+ and AC133+ cells during ex vivo expansion. Selection of the expanded progenitor cells without apoptosis may be useful for the hematopoietic stem cell transplantation.
- All Author(s)
- C. K. Kim
; N. S. Lee
; S. B. Bae
; K. T. Lee
; S. K. Park
; K. H. Jang
; H. J. Cheong
; S. J. Kim
; J. H. Won
; H. S. Park
; D. S. Hong
- Issued Date
- 2004
- Type
- Article
- Keyword
- Apoptosis; Fas antigen; Bax; ex vivo expansion; Hematopoietic progenitor cells
- Publisher
- 대한혈액학회
Korean Society of Hematology
- ISSN
- 1225-0546
- Citation Title
- 대한혈액학회지
The Korean journal of hematology
- Citation Volume
- 39
- Citation Number
- 2
- Citation Start Page
- 95
- Citation End Page
- 102
- Language(ISO)
- kor
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/3212
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