A 3D-printed polycaprolactone/β-tricalcium phosphate mandibular prosthesis: A pilot animal study
- Abstract
- OBJECTIVE: In this study, we assessed the effectiveness of a tonsil-derived mesenchymal stem cell (TMSC)-transplanted polycaprolactone/beta-tricalcium phosphate prosthesis (specifically designed for easier fixing and grafting with a single scaffold) on rabbit mandible osteogenesis. METHODS: The mandibles of 18 rabbits were exposed, and 10 × 8-mm bone defects were made. Two rabbits did not receive implants; four were reconstructed with the scaffold control (SC) (SC group); four were reconstructed with scaffolds soaked in peripheral blood (PB) (PB group); four were reconstructed with TMSC-transplanted scaffolds (TMSC group); and four were reconstructed with differentiated osteocyte-transplanted scaffolds (DOC) (DOC group). Each rabbit was sacrificed 12 weeks after surgery, and the area of new bone formation was investigated by mechanical testing, histology, and micro-computed tomography. RESULTS: More extended and denser new bone masses were observed in the TMSC and DOC groups, although fibrosis and vascular formation levels were similar in all groups, suggesting that the dual-structured scaffold alone provides a good environment for bone attachment and regeneration. The bone volumes of representative scaffolds from the SC, PB, TMSC, and DOC groups were 43.12, 48.35, 53.10, and 57.44% of the total volumes, respectively. CONCLUSION: The design of the scaffold resulted in effective osteogenesis, and TMSCs showed osteogenic potency, indicating that their combination could enable effective bone regeneration. LEVEL OF EVIDENCE: NA Laryngoscope, 130:358-366, 2020.
- All Author(s)
- J. H. Park
; S. Y. Jung
; C. K. Lee
; M. J. Ban
; S. J. Lee
; H. Y. Kim
; H. J. Oh
; B. K. Kim
; H. S. Park
; S. H. Jang
; H. S. Kim
- Issued Date
- 2020
- Type
- Article
- Keyword
- Animals; Calcium Phosphates/*chemistry; Compressive Strength; Male; *Mandibular Prosthesis; Mesenchymal Stem Cell ransplantation; Osteocytes/transplantation; Osteogenesis; Pilot Projects; Polyesters/*chemistry; *Printing, Three-Dimensional; Prosthesis Design; Prosthesis Fitting; Rabbits; Tissue Scaffolds; X-Ray Microtomography; 3D printing; Graft material; Stem cell
- ISSN
- 0023-852x
- Citation Title
- The Laryngoscope
- Citation Volume
- 130
- Citation Number
- 2
- Citation Start Page
- 358
- Citation End Page
- 366
- Language(ISO)
- eng
- DOI
- 10.1002/lary.27908
- URI
- http://schca-ir.schmc.ac.kr//handle/2022.oak/367
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