담관 결찰 쥐의 간세포와 담관세포의 anion exchanger와 CFTR 발현
- Alternative Title
- Expression of Anion Exchanger and CFTR in the Hepatocyte and Cholangiocytes in Bile Duct-Ligated Rat
- Abstract
- Cystic fibrosis transmembrane conductance regulator (CFTR) gene and sodium-independent $Cl^-/HCO_3^-$ anion exchanger (AE) genes are expressed in a wide variety of mammalian tissues including cholangiocytes. They play an important role in the regulation of intracellular pH (pHi) as well as in transepithelial acid/base transport necessary for biliary bicarbonate secretion. The aim of this study was to examine the expression level of CFTR gene and AE genes (AE1, AE2 and AE3) in the cholangiocytes and the hepatocytes, and also measure AE2 gene expression level after bile duct ligation (BDL). As we previously described, isolated hepatocytes and cholangiocytes from the liver of normal and BDL rats were prepared and gene expression levels were measured by using RT-PCR. We found that AE1, AE2, and AE3 genes were expressed in both hepatocytes and cholangiocytes, but CFTR was only in cholangiocytes. AE2 gene expression level was higher in the BDL hepatocytes than normal hepatocytes, which was significantly different between two groups. AE2 gene expression level was lower in the BDL cholangiocytes than normal cholangiocytes. However, AE2 gene expression level in both hepatocytes and cholangiocytes were not changed with a longer duration of BDL. These results suggest that CFTR and AE2 may play an important role in the pathogenetic mechanism of biliary cholestatic liver disease.
- All Author(s)
- 이재동
; 왕준호
; 기승석
; 최원혁
; 박재승
; 조원규
; 박정준
; 김홍수
- Issued Date
- 2011
- Type
- Article
- Keyword
- Hepatocyte; cholangiocyte; CFTR; anion exchanger; cholestasis
- ISSN
- 1225-0023
; 1225-9918
; 2287-3406
- Citation Title
- Journal of Life Science
- Citation Volume
- 21
- Citation Number
- 12
- Citation Start Page
- 1772
- Citation End Page
- 1777
- Language(ISO)
- kor
- DOI
- 10.5352/JLS.2011.21.12.1772
- URI
- http://schca-ir.schmc.ac.kr//handle/2022.oak/428
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