Association analysis of thromboxane A synthase 1 gene polymorphisms with aspirin intolerance in asthmatic patients
- Abstract
- Aim: Thromboxane A synthase (TBXAS1) converts prostaglandin H to thromboxane A, a potent constrictor of smooth respiratory muscle. Thus, functional alterations of the TBXAS1 gene may contribute to aspirin-intolerant asthma (AIA). Materials & methods: We investigated the relationship between SNPs in the TBXAS1 gene and AIA. Asthmatics (n = 470) were categorized into AIA (20% or greater decreases in forced expiratory volume in 1 s [FEV1], or 15% to 19% decreases in FEV1 with naso-ocular or cutaneous reactions) and aspirin-tolerant asthma (ATA). A total of 101 SNPs were genotyped. mRNA expression of the TBXAS1 gene by peripheral blood mononuclear cells and plasma thromboxane B2 (TXB2) concentrations were measured by reverse transcriptase (RT)-PCR and ELISA. Results: Logistic regression analysis showed that the rare allele frequency of rs6962291 in intron 9 was significantly lower in the AIA group (n = 115) than in the ATA group (n = 270) (p(corr) = 0.04). The linear regression analysis revealed a strong association of rs6962291 with the aspirin challenge-induced FEV1 fall (p = 0.003). RT-PCR revealed an exon-12-deleted splice variant. We measured TBXAS1 mRNA levels in peripheral blood mononuclear cells. The mRNA levels of the full-length wild-type and splice variant were significantly higher in the TT homozygotes than in the AA homozygotes of rs6962291 (1.00 +/- 0.18 vs 0.57 +/- 0.03 and 1.00 +/- 0.18 vs 0.21 +/- 0.05, p = 0.047 and 0.001, respectively). The plasma TXB2 level was significantly lower in rs6962291 AA carriers than in rs6962291 IT (p = 0.016) carriers. Conclusion: The rare allele of rs6962291 may play a protective role against aspirin hypersensitivity via a lower catalytic activity of the TBXAS1 gene, attributed to the increase of a nonfunctioning isoform of TBXAS1.
- All Author(s)
- S. H. Oh
; Y. H. Kim
; S. M. Park
; S. H. Cho
; J. S. Park
; A. S. Jang
; S. W. Park
; S. T. Uh
; Y. M. Lee
; M. K. Kim
; I. S. Choi
; C. S. Hong
; Y. W. Lee
; J. Y. Lee
; B. W. Choi
; B. L. Park
; H. D. Shin
; C. S. Park
- Issued Date
- 2011
- Type
- Article
- Keyword
- aspirin; asthma; gene; isoform; SNP; thromboxane A synthase 1
- ISSN
- 1462-2416
- Citation Title
- Pharmacogenomics
- Citation Volume
- 12
- Citation Number
- 3
- Citation Start Page
- 351
- Citation End Page
- 363
- Language(ISO)
- eng
- DOI
- 10.2217/pgs.10.181
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/856
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