Urinary exosomal microRNA profiling in type 2 diabetes patients taking dipeptidyl peptidase-4 inhibitor compared with sulfonylurea
- Abstract
- BACKGROUND: Dipeptidyl peptidase-4 (DPP-4) inhibitor has been reported to have kidney-protective benefits. To elucidate how antidiabetic agents prevent diabetic kidney disease progression, it is important to investigate their effect on the kidney environment in type 2 diabetes mellitus (DM) patients. Herein, we investigated the expression pattern of urinary exosome-derived microRNA (miRNA) in patients taking a combination of DPP-4 inhibitor and metformin (DPP-4 inhibitor group) and compared them with patients taking a combination of sulfonylurea and metformin (sulfonylurea group). METHODS: This was a prospective study involving 57 patients with type 2 DM (DPP-4 inhibitor group, n = 34; sulfonylurea group, n = 23) and healthy volunteers (n = 7). We measured urinary exosomal miRNA using the NanoString nCounter miRNA array (NanoString Technologies) across the three groups (n = 4 per each group) and validated findings using real-time polymerase chain reaction. RESULTS: Twenty-one differentially expressed candidate miRNAs were identified, and six (let-7c-5p, miR-23a-3p, miR-26a-3p, miR-30d, miR-205, and miR-200a) were selected for validation. Validation showed no significant difference in miRNA expression between the DPP-4 inhibitor and sulfonylurea groups. Only miR-23a-3p was significantly overexpressed in the diabetes group compared with the control group (DPP-4 inhibitor vs. control, p = 0.01; sulfonylurea vs. control, p = 0.007). This trend was consistent even after adjusting for age, sex, and body mass index. CONCLUSION: There was no significant difference in urine exosome miRNA expression between diabetic participants taking DPP-4 inhibitor and those taking sulfonylurea. The miR-23a levels were higher in diabetic participants than in nondiabetic controls.
- All Author(s)
- N. J. Cho
; D. Y. Kim
; S. H. Kwon
; T. W. Ha
; H. K. Kim
; M. R. Lee
; S. W. Chun
; S. Park
; E. Y. Lee
; H. W. Gil
- Issued Date
- 2021
- Type
- Article
- Keyword
- Biomarkers; Diabetes mellitus; Diabetic nephropathies; Exosomes; MicroRNAs; Type 2
- Publisher
- 대한신장학회
- ISSN
- 2211-9132
; 2211-9140
- Citation Title
- Kidney research and clinical practice
- Citation Volume
- 40
- Citation Number
- 3
- Citation Start Page
- 383
- Citation End Page
- 391
- Language(ISO)
- eng
- DOI
- 10.23876/j.krcp.21.015
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/957
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